Agilent webinář: Size Exclusion Chromatography of Biomolecules: Column Selection and Method Optimization

Size exclusion chromatography (SEC) is a noninteractive technique that separates solutes according to their molecular size in non-denaturing conditions. It is often used as the first step in isolation of a protein from a crude sample. SEC is a gold standard to determine aggregate and fragment impurities in biotherapeutics. 

In this webinar, we will outline basic theory, principles, and practical guidelines on column selection for SEC method development. We will overview biotherapeutic aggregation and how method parameters such as flow rate, column length, injection volume, and pH and salt concentration influence SEC.