Phase Holographic Imaging provides a non-invasive, innovative tool that lets researchers continuously image, monitor and, quantitatively analyze both single and populations of cells directly inside your incubator without any labels or stains.
The HoloMonitor® M4 is a compact microscope designed to withstand continuous digital holography imaging, inside a normal incubator and record your cells in real-time.
The generated results are non-invasive real 3D images and quantitative data on cell cultures, all the way to single-cell analysis.
With its small foot-print and ease of use, the HoloMonitor® M4 has deemed a staple in every lab working with cell cultures for obtaining real-time, time-lapse quantitative data as well as 3D images and/or movies of your cell samples.
Features
The Holomonitor AppSuite Software
The AppSuite Software is an integrated solution featuring different application modules including Guided End-point Assays for a quick assessment of cell count and cell culture quality, Guided Kinetic Assays for publication-ready kinetic results with minimum effort, and In-dept Analysis Assays that provide single cell tracking for the analysis of individual cell and/or cell populations. The HoloMonitor label-free live cell imaging system offers a powerful and unique portfolio of cell biological applications. All applications are designed for adherent cells, except for the Cell Counter which offers automatic counting of cells in suspension. Please see the full list of AppSuite Assays below:
Guided End-Point Assays
Cell Counting
Cell Quality Control
Guided Kinetic Assays
Kinetic Cell Proliferation
Kinetic Cell Motility
Kinetic Dose Response
Wound Healing
In-depth Analysis Assays
Single-cell Tracking
Cell Morphology
App Suite Workflow
Choose Application - Basic Setup - Capture Setup - Capture & Analysis - Result Presentation
- Select your biological application: cell count, cell QC, kinetic cell proliferation, kinetic cell motility or kinetic dose response.
- Choose culture vessel and experiment conditions: selected microslides, 6-well, 24-well or 96-well plates are recommended.
- Choose experiment duration and frequency of image acquisition. Inspect images to ensure optimal image quality.
- The system automatically captures time-lapse image sequences. While capturing, the software analyses the images by automatic identification of individual cells and extraction of relevant output data.
- The relevant results (graphs, tables, images etc) are immediately provided to the user and can easily be exported to Excel.
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Technology
Label-free Live Cell Imaging & Analysis
Label-free live cell imaging and analysis is a transformative technology that enables researchers to observe living cells in their natural state, without the use of dyes, stains, or fluorescent markers. This non-invasive approach preserves cellular integrit...
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